254960.fig.005a
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254960.fig.005b
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Figure 5: Effects of SLE and diosgenin on WEHI-3 cells in the intrinsic apoptotic pathway. Cells were pretreated with specific inhibitor of caspases-9 (z-LEHD-fmk) for 1 h after exposure to SLE (200 μg/mL) or diosgenin (50 μM) for 12, 24, 36, and 48 h. (a) The whole-cell lysates were subjected to caspase-9 activity assay and (b) cells were collected after SLE or diosgenin for a 48 h treatment to determine the percentage of viable cells. Data are presented as the mean ± S.E.M. of three independent experiments. *, , significantly different compared with SLE treatment. (c) The reactive oxygen species (ROS) of SLE- or diosgenin-treated WEHI-3 cells from each time point were measured by staining with H2DCF-DA. (d) The mitochondrial membrane potential ( ) of both reagents-treated WEHI-3 cells was measured by staining with DiOC6. Data are presented as the mean ± S.E.M. of three independent experiments. *, , significantly different compared with 0 h treatment.