Ginseng and Its Active Components Ginsenosides Inhibit Adipogenesis in 3T3-L1 Cells by Regulating MMP-2 and MMP-9
Figure 4
Zymographic analysis of 3T3-L1 cells. (a) 3T3-L1 cells were treated with MDI (control), MDI plus GE (10 g/mL), TGSs (10 g/mL), or individual GSs (i.e., Rb1, Rb2, Rc, Rd, Rf, Rg1, Rg2, Rg3 (10 M each), and Re (0.1 M)). Protein extracts from 3T3-L1 cells were applied to a gelatin-containing gel. Gelatinolytic activity was measured by zymography. (b) MMP-2 and MMP-9 activities were quantified by densitometric analyses. All values are expressed as the mean ± SD. # compared with the ND group, * compared with the control group. M: protein molecular weight marker; ND: nondifferentiated; Con: differentiated control; GE: ginseng extract; TGSs: total ginsenosides.
