The Crude Extract from Puerariae Flower Exerts Antiobesity and Antifatty Liver Effects in High-Fat Diet-Induced Obese Mice

Table 2

Polymerase chain reaction primer sequences.

Gene name	Function	GenBank ID	Direction	Sequences
Fatty acid synthase (FAS)			Forward	TCCTGGGAGGAATGTAAACAGC
	Lipogenesis	NM_007988.3
			Reverse	CACAAATTCATTCACTGCAGCC
Acetyl-CoA carboxylase (ACC)			Forward	TGGATCCGCTTACAGAGACTTT
	Lipogenesis	NM_133904.2
			Reverse	GCCGGAGCATCTCATTCG
Carnitine palmitoyltransferase l (CPT1)			Forward	CTTCCAAGGCAGAAGAGTGG
	Beta-oxidation	NM_013495.2
			Reverse	GAACCTTGGCTGCGGTAAGAC
Medium-chain acyl dehydrogenase (MCAD)			Forward	TCGAAAGCGGCTCACAAGCAG
	Beta-oxidation	NM_007382.4
			Reverse	CACCGCAGCTTTCCGGAATGT
Acyl-CoA oxidase (ACO)			Forward	TCTTCTTGAGACAGGGCCCAG
	Beta-oxidation	AF006688.1
			Reverse	GTTCCGACTAGCCAGGCATG
Hormone-sensitive lipase (HSL)			Forward	CCTACTGCTGGGCTGTCAA
	Lipolysis	BC021642.1
			Reverse	CCATCTGGCACCCTCACT
Uncoupling protein 1 (UCP1)			Forward	CTGGGCTTAACGGGTCCTC
	Thermogenesis	NM_009463.2
			Reverse	CTGGGCTAGGTAGTGCCAGTG
PPARg coactivator alpha (PGCla)			Forward	TCGATGTGTCGCCTTCTTGC
	Mitochondriogenesis	BC066868.1
			Reverse	ACGAGAGCGCATCCTTTGG
GAPDH			Forward	ATGACATCAAGAAGGTGGTG
	Housekeeping	XM_001478412.1
			Reverse	CATACCAGGAAATGAGCTTG