Research Article

Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest

Figure 6

The effect of Formula 14 on NSCLC cell lines H460 and H520. Survival rate of H460 (a) and H520 (b) cells following treatment with Formulae14 for 72 hr was analyzed by MTT assay. (c) Formula 14 decreased the colony formation activity of H460 cells. Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (d) Western blots of the cell cycle regulatory proteins in H520 cells after 72 hr treatment of Formula 14. Results of 3 independent experiments were shown at the same time to show the consistent results.
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