Myrrh Inhibits LPS-Induced Inflammatory Response and Protects from Cecal Ligation and Puncture-Induced Sepsis
Effects of myrrh on MAPKs activation. (a) Peritoneal macrophages ( mm dish) were treated with myrrh (0.5 mg/mL) for 1 h, then stimulated with LPS (100 ng/mL) as indicated time points. The cells were harvested for western blot. Total cellular proteins (20 μg) were resolved by SDS-PAGE, transferred to PVDF membrane, and detected with phosphospefic ERK 1/2 ( kDa), JNK ( kDa), and p38 (38 kDa) antibody, as described in Subjects and Methods. ERK, JNK, and p38 were used as loading control. The cells were treated with SP600125, JNK inhibitor as indicated dose for 1 h, then stimulated with LPS for 24 h. (b) The expression of iNOS was detected by Western blot, then nitrite production was measured by Griess method. Actin was used as loading control. (c) The production of PGE2 and expression of COX-2 were measured by ELISA and Western blot, respectively. Actin was used as loading control. (d) Production of TNF-α was examined by ELISA. Details are described in Subjects and Methods. A representative western blot of three experiments is shown. The values are means ± SD of three independent experiments. * versus DMSO. + versus LPS.