The Chinese Herbal Decoction Danggui Buxue Tang Inhibits Angiogenesis in a Rat Model of Liver Fibrosis
Effect of DBT and NAC on α-SMA protein expression in CCl4-induced fibrotic liver tissue in rats. (a) Immunohistochemical staining, DAB × 200. Normal liver tissue had little positive staining. Tissue from the model group had much stronger and more extensive positive staining among fibrotic septa and in the sinusoidal area. DBT and NAC significantly decreased the amount of positive staining. (b) A representative western blot. Consistent with the immunohistochemistry findings, the semi-quantitative value of the α-SMA western blot was significantly increased in fibrotic livers. Compared to the model control group, DBT significantly decreased α-SMA expression, and the effect of DBT was more pronounced than that of NAC. (c) A histogram plot showing the densitometric analysis corresponding to the mean ± SD of three independent experiments. versus normal group, versus model group, versus NAC group.