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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 294528, 11 pages
Research Article

Pilot Study with regard to the Wound Healing Activity of Protein from Calotropis procera (Ait.) R. Br.

Infectious Diseases Programme, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

Received 5 March 2012; Revised 16 May 2012; Accepted 28 May 2012

Academic Editor: Fatma U. Afifi

Copyright © 2012 Ramar Perumal Samy and Vincent T. K. Chow. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We provide the scientific basis for the use of Calotropis procera for treating skin and wound infections in traditional medicine. The aqueous extract of stem-bark of C. procera exhibited more pronounced potent antimicrobial activity. Calo-protein was purified and identified from the most-active aqueous extracts of C. procera and showed broad-spectrum activity. Calo-protein inhibited the growth of S. aureus and E. aerogenes effectively at 25 μg/ml concentration. Mice topically treated with Calo-protein revealed significant wound healing after 14 days comparable to fusidic acid (FA) as positive control. This protein was devoid of cytolytic effect even at higher concentrations on skin cells after 24 h. Further investigation of this Calo-protein of C. procera on bacterial inhibition may provide a better understanding of the scientific basis and justification for its use in traditional medicine.