Pilot Study with regard to the Wound Healing Activity of Protein from Calotropis procera (Ait.) R. Br.

Table 1

Antimicrobial activity of different extracts of stem-bark of C. procera tested on Gram-positive and Gram-negative bacteria at 100 𝜇g/mL concentration and their cytotoxic potential was evaluated against human macrophage (U-937) cell lines by XTT assay at 2000, 1000, 500, 250, 100, 50, 25, and 12.5 𝜇g/mL doses.

Crude extracts	Phytochemical screening	Yield	E. coli	E. aerogenes	P. vulgaris	P. mirabilis	P. aeruginosa	S. aureus	Cytotoxicity (𝜇g/mL)
(1) Hexane	Flovanoids	5.36 gm	—	11±0.94	7±0.1	9±0.06	—	8±0.12	<250
(2) Ethyl acetate	Steroids	3.42 gm	—	18±0.06	17±0.27	15±0.2	—	25±0.06	<500
(3) Dichloromethane	Polyphenols	4.78 gm	—	12±0.4	8±0.23	11±0.48	—	13±0.21	<500
(4) Methanol	Terpenoids	5.98 gm	14±0.31	19±0.2	23±0.4	20±0.6	8±0.12	27±0.06	>2000
(5) Aqueous	Glycosides; Tannins; saponins	7.15 gm	11±0.14	22±0.7	25±1.3	20±0.12	9±0.23	28±0.15	>2000
(6) Chloramphenicol	Standard antibiotic	30 𝜇g	21±0.34	24±0.3	23±0.12	26±0.23	10±0.35	29±0.12	—

Clinical isolates of Gram-positive and Gram-negative bacteria were used. Values are mean ± SD of three replicates (𝑛=3), inhibition zones in diameters (mm) produced by the extract around the discs. Size of disc was 6 mm. Absence of bacterial inhibition denoted by (—), that is, no inhibition zone around the disc by crude extract of plants.