Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells
Inhibition of proliferation, migration and invasion of endothelial cells after treatment with EENL. HUVECs were treated for 24 hours with 10.0 to 80.0 μg/mL of EENL or vehicle as control. (a) The antiproliferative effect of EENL was evaluated using the MTS viability assay. (b) Cell migration and invasion were assessed using fibronectin and Matrigel-coated FluoroBlock 24-multiwell insert plates, respectively. Experiments were performed in triplicate and the data were expressed as the mean ± SD of the triplicate determinations of a representative experiment in % cell viability of vehicle-treated cells (100%) (*).