Research Article

Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells

Figure 3

Inhibition of proliferation, migration and invasion of endothelial cells after treatment with EENL. HUVECs were treated for 24 hours with 10.0 to 80.0 μg/mL of EENL or vehicle as control. (a) The antiproliferative effect of EENL was evaluated using the MTS viability assay. (b) Cell migration and invasion were assessed using fibronectin and Matrigel-coated FluoroBlock 24-multiwell insert plates, respectively. Experiments were performed in triplicate and the data were expressed as the mean ± SD of the triplicate determinations of a representative experiment in % cell viability of vehicle-treated cells (100%) (* ).