Research Article

MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D3 in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells

Figure 3

Western blot analysis for the expressions of p21 and p27 after treating MCF-7 cells with 1α25(OH)2D3 and MART-10. (a) A western blot (30 μg protein was loaded for each individual lane) depicting a typical dose-dependent upregulation of p21 protein expression in response to the treatment with 1α25(OH)2D3 or MART-10 for 2 days (upper panel). Actin was used as the loading control. The lower panel shows the average radio of the dose-dependent p21 expression relative to actin expression from three independent experiments. Each value is a mean ± SD of three independent determinations. (b) A western blot (30 μg protein was loaded for each individual lane) depicting a typical dose-dependent up-regulation of p27 protein expression in response to the treatment with 1α25(OH)2D3 or MART-10 for 2 days (upper panel). Actin was used as the loading control. The lower panel depicts the average radio of the dose-dependent p27 expression relative to actin expression from three independent experiments. Each value is a mean ± SD of three independent determinations. , versus control.
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