Research Article

MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D3 in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells

Figure 4

Effects of 1α25(OH)2D3 and MART-10 on MCF-7 cell apoptosis analyzed by flow cytometry with Annexin V-FITC, PI staining, and TUNEL assay. (A) Annexin V-FITC in conjunction with PI staining was used to distinguish early apoptotic (Annexin V-FITC positive, PI negative; bottom right quadrant of each panel) from late apoptotic or necrotic cells (Annexin V-FITC positive, PI positive; top right quadrant of each panel). Fluorescence intensity for Annexin V-FITC is plotted on the x-axis, and PI is plotted on the y-axis. (B) The apoptotic effects induced by MCF-7 cells were analyzed by TUNEL assay to measure the extent of DNA fragmentation visualized by fluorescence microscopy: (a) control; (b) cells treated with 10−7 M MART-10; (c) cells treated with 10−7 M 1α25(OH)2D3. The cells showing positive DNA fragmentation were circled; (d) relative apoptotic index. Each value represents the average of three determinations. , versus control.
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