Reversal of the Caspase-Dependent Apoptotic Cytotoxicity Pathway by Taurine from Lycium barbarum (Goji Berry) in Human Retinal Pigment Epithelial Cells: Potential Benefit in Diabetic Retinopathy
Figure 3
Effect of LB extract and taurine on high-glucose-induced ARPE-19 cells, with condensed and/or fragmented chromatin determined by the fluorescent dye Hoechst 33342 method. (a) Quantification of apoptotic cells in control and in high-glucose-induced ARPE-19 cells treated in the absence and presence of LB extract (0.1, 0.5, and 0.75 mg/mL) or taurine (0.001, 0.1, and 1 mM) for 48 h. Positive controls (RG and PG) and the osmotic control (M) were also used. The results are presented as percentage of total number of cells counted (nonapoptotic + apoptotic) and represent the mean ± SEM ( independent experiments). * versus HG; # versus control. (b) Morphological analysis of control (panel 1) and high-glucose-treated ARPE-19 cells with Hoechst staining. Arrows indicate examples of cells with condensed and/or fragmented nuclei. Scale bar 50 μm.
