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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 342652, 8 pages
http://dx.doi.org/10.1155/2012/342652
Research Article

In Vitro Cytotoxic Potential of Essential Oils of Eucalyptus benthamii and Its Related Terpenes on Tumor Cell Lines

1Postgraduate Program in Pharmaceutical Sciences, Federal University of Paraná, 632 Prefeito Lothário Meissner Avenida, 80210-170 Curitiba, PR, Brazil
2Research Center for Chemistry, Biology and Agriculture, University of Campinas, P.O. Box 6171, 13081-970 Campinas, SP, Brazil
3Department of Chemistry, Federal University of Paraná, Polytechnic Center, P.O. Box 19081, 81531-990 Curitiba, PR, Brazil
4Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenida, 84030-900 Ponta Grossa, PR, Brazil
5Department of General Biology, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenida, 84030-900 Ponta Grossa, PR, Brazil

Received 21 December 2011; Revised 21 February 2012; Accepted 24 February 2012

Academic Editor: Y. Ohta

Copyright © 2012 Patrícia Mathias Döll-Boscardin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Eucalyptus L. is traditionally used for many medicinal purposes. In particular, some Eucalyptus species have currently shown cytotoxic properties. Local Brazilian communities have used leaves of E. benthamii as a herbal remedy for various diseases, including cancer. Considering the lack of available data for supporting this cytotoxic effect, the goal of this paper was to study the in vitro cytotoxic potential of the essential oils from young and adult leaves of E. benthamii and some related terpenes ( -pinene, terpinen-4-ol, and -terpinene) on Jurkat, J774A.1 and HeLa cells lines. Regarding the cytotoxic activity based on MTT assay, the essential oils showed improved results than -pinene and -terpinene, particularly for Jurkat and HeLa cell lines. Terpinen-4-ol revealed a cytotoxic effect against Jurkat cells similar to that observed for volatile oils. The results of LDH activity indicated that cytotoxic activity of samples against Jurkat cells probably involved cell death by apoptosis. The decrease of cell DNA content was demonstrated due to inhibition of Jurkat cells proliferation by samples as a result of cytotoxicity. In general, the essential oils from young and adult leaves of E. benthamii presented cytotoxicity against the investigated tumor cell lines which confirms their antitumor potential.