In Vitro Cytotoxic Potential of Essential Oils of Eucalyptus benthamii and Its Related Terpenes on Tumor Cell Lines

Table 3

Evaluation of cytotoxicity by MTT assay in cell lines after 72 h.

Cell line	IC50 (μg/mL)
	YLEO	ALEO	α-pinene	γ-terpinene	terpinen-4-ol
Jurkat	56.51±1.48	36.63±3.52	186.09±37.98	156.92±10.22	54.84±3.85
J774A.1	166.87±5.77	178.85±6.11	>300	>300	189.7±14.74
HeLa	120.57±6.22	101.90±19.47	>300	>300	>300

Legend: YLEO: young leaves essential oil; ALEO: adult leaves essential oil; IC₅₀: concentration that reduces the mitochondrial activity by 50%. The results are shown as mean ± SD from three independent experiments.