Research Article

Anti-Hepatitis B Virus Effect and Possible Mechanism of Action of 3,4-O-Dicaffeoylquinic Acid In Vitro and In Vivo

Table 4

Anti-HBV activity of 3,4-O-dicaffeoylquinic acid in HepG2.2.15 cells. (After the cells were treated with the test compound for 4 days.)

GroupsConcentration (μg/mL)HBsAgHBeAg
AbsorbencyInhibition (%)AbsorbencyInhibition (%)

Vehicle
Lamivudine100 10.01
50 6.67
10
3,4-Dicaffeoylquinic acid100 50.23 29.77
50 28.50 15.02
10 5.48 6.70

Lamivudine was used as the positive control in the anti-HBV assay. 0.1% DMSO was used as the vehicle control. Inhibition (%) = (the mean absorbency value in negative control group − the mean absorbency value in experimental group)/(the mean absorbency value in negative control group) × 100%. Data are expressed as the means ± SD of three independent experiments. * and ** compared with the vehicle group.