Inhibitory Effects of Daiokanzoto (Da-Huang-Gan-Cao-Tang) on P-Glycoprotein

<table>Lineweaver-Burk (a) and Dixon (b) plots for the inhibitory effects of rhubarb on ATPase activity in the presence of verapamil. (a) The final concentration of rhubarb was 0 (●), 0.0625 (○), 0.25 (▲), or 0.5 (<svg height="10.6625" id="M42" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.4125 10.6625" width="11.4125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.6625)">
<g transform="translate(72,-63.47)">
<text transform="matrix(1,0,0,-1,-71.95,63.52)">
<tspan style="font-size: 12.50px; " x="0" y="0">Δ</tspan>
</text>
</g>
</g>
</svg>) mg/mL. (B) The final concentration of verapamil was 1.56 (●), 3.13 (○), 6.25 (▲), 12.5 (<svg height="10.6625" id="M43" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.4125 10.6625" width="11.4125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.6625)">
<g transform="translate(72,-63.47)">
<text transform="matrix(1,0,0,-1,-71.95,63.52)">
<tspan style="font-size: 12.50px; " x="0" y="0">Δ</tspan>
</text>
</g>
</g>
</svg>), 25 (■), or 50 (□) <svg height="9.6750002" id="M44" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.675)">
<g transform="translate(72,-64.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>M. Data represent the mean <svg height="9.3249998" id="M45" style="vertical-align:-1.09097pt" version="1.1" viewbox="0 0 10.825 9.3249998" width="10.825" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.325)">
<g transform="translate(72,-64.54)">
<text transform="matrix(1,0,0,-1,-71.95,65.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">±</tspan>
</text>
</g>
</g>
</svg> SD of three experiments. <svg height="10.575" id="M46" style="vertical-align:-0.20064pt" version="1.1" viewbox="0 0 12.625 10.575" width="12.625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.575)">
<g transform="translate(72,-63.54)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑉</tspan>
</text>
</g>
</g>
</svg>: ATPase activity (nmol/mg protein/min), <svg height="10.725" id="M47" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 11.375 10.725" width="11.375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.725)">
<g transform="translate(72,-63.42)">
<text transform="matrix(1,0,0,-1,-71.95,63.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑆</tspan>
</text>
</g>
</g>
</svg>: verapamil concentration (<svg height="9.6750002" id="M48" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.675)">
<g transform="translate(72,-64.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>M).</table>

Evidence-Based Complementary and Alternative Medicine

fig6

Figure 6

Figure 6: Inhibitory Effects of Daiokanzoto (Da-Huang-Gan-Cao-Tang) on P-Glycoprotein 