Ganoderma tsugae Induces S Phase Arrest and Apoptosis in Doxorubicin-Resistant Lung Adenocarcinoma H23/0.3 Cells via Modulation of the PI3K/Akt Signaling Pathway
Figure 3
Effect of GTE on cell cycle distribution in H23/0.3 cells. (a) H23/0.3 cells were treated with GTE (0.3 mg/mL) for 16 and 24 h. The cell cycle distribution was measured by flow cytometry as described in Section 2. The error bars indicate the standard deviations of each phase (%), which were the means of three independent experiments. (b) H23/0.3 cells were treated with various concentrations of GTE (0 mg/mL, 0.3 mg/mL, and 0.9 mg/mL) for 16 h and 24 h. The expression of S phase regulators was determined by Western blotting as described in Section 2. (c) A histogram showing the relative protein levels from (b). Results are expressed as the mean ± SD of three independent experiments.
