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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 375671, 7 pages
Research Article

Wound Healing and Anti-Inflammatory Effect in Animal Models of Calendula officinalis L. Growing in Brazil

1Laboratory of Natural Products, Pharmacy Faculty, Federal University of Goiás, 74605-220 Goiânia, GO, Brazil
2Department of General Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, 74605-050 Goiânia, GO, Brazil
3Veterinary School, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil

Received 1 October 2011; Accepted 14 October 2011

Academic Editor: Esra Küpeli Akkol

Copyright © 2012 Leila Maria Leal Parente et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Calendula officinalis is an annual herb from Mediterranean origin which is popularly used in wound healing and as an anti-inflammatory agent. In this study, the ethanolic extract, the dichloromethane, and hexanic fractions of the flowers from plants growing in Brazil were produced. The angiogenic activity of the extract and fractions was evaluated through the chorioallantoic membrane and cutaneous wounds in rat models. The healing activity of the extract was evaluated by the same cutaneous wounds model through macroscopic, morphometric, histopathologic, and immunohistochemical analysis. The antibacterial activity of the extract and fractions was also evaluated. This experimental study revealed that C. officinalis presented anti-inflammatory and antibacterial activities as well as angiogenic and fibroplastic properties acting in a positive way on the inflammatory and proliferative phases of the healing process.