Xiaoyaosan Decoction Regulates Changes in Neuropeptide Y and Leptin Receptor in the Rat Arcuate Nucleus after Chronic Immobilization Stress

Figure 6

RT-qPCR detection of NPY mRNA and ob-R mRNA in the ARC. To calculate the relative amount of NPY or ob-R gene expression the formula (ΔCT=CT_targetgene−CT_{internalreference}) is used and analyzed with 2^−ΔCT for statistics. Each bar represent the mean values and vertical bars represent SEM. ^⋇⋇𝑃<0.01 as compared with the control group; ^▲𝑃<0.05 or ^▲▲𝑃<0.01 as compared with the 21-day stress group.

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