Figure 4: Expression of TRAP mRNA. (a) TRAP mRNA expression was assessed by RT-PCR, and GAPDH was used as control. (b) Melt curve analysis of TRAP and GAPDH. It demonstrates the single product-specific melting temperatures: TRAP (83.3°C), GAPDH (80.8°C). No primer-dimer formation was produced during 40 amplification cycles. The images were representative the littermates of the same litter from the four groups.