Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

<table>Effect of BSA on sphingomyelin hydrolysis. (a) Sphingomyelin and (b) dihydrosphingomyelin standards (1,000 pmol) were hydrolyzed with sphingolipid ceramide <i>N</i>-deacylase (SCDase) plus sphingomyelinase (SMase) in a reaction buffer with varying concentrations of fatty acid-free BSA (0, 7.5, 15, and 30%). The released sphingoid bases were derivatized with OPA and analyzed by HPLC. Values are expressed as the mean ± SD (<svg height="10.925" id="M13" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 10.925" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> were statistically significant.</table>

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fig4

Figure 4

Figure 4: Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis 