Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

Table 3

Levels of sphingomyelin in plasma and tissues from mice and cultured cells.

Source	Sphingomyelin^a
ICR mice
Plasma	407.40±0.31
Brain	55.60±0.43
Kidney	43.75±0.21
Liver	22.26±0.14
Cell type
3T3-L1 cells	60.10±0.24
HT-29 cells	58.38±0.37
RASMCs	62.69±0.08

^aValues are expressed as the mean ± S.D. (𝑛=3) in μM for mouse plasma and pmol/μg protein for mouse tissues and cultured cells. RASMCs: rat aortic smooth muscle cells.