Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 5

Research Article

Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway

Figure 5

Disruption of actin filament formation by PS-A in RCC cells. (a) 2 × 105 of 786O cells were treated with 0, 0.3, and 0.6 mg/mL of PS-A for 12 h. After fixation, cells were stained with Alexa568-phalloidin (red) and Hoechst 33258 (blue). Fluorescent images were taken with a Leica TCS SP5 Spectral Confocal System. Bars, 20 μm. (b) 2 × 105 of 786O cells were treated with or without 0.3 mg/mL of PS-A, and the fractions containing F- or G-actin were separated and then subjected to Western blotting using actin antibody. The intensities of bands were quantitated, and the ratio of F-actin to G-actin was calculated. The data are represented as the means ± SD ( ). * compared with untreated cells.

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