In Vitro and In Vivo Genotoxicity Assessment of Aristolochia manshuriensis Kom.
Table 2
Effects of Arisolochiae manshuriensis Kom. (AMK) on the chromosomal aberration in Chinese hamster ovary (CHO)-K1 cells.
Treatment (μg/mL)
AA I/ II (μg/mL)
S9 mix
Time (h)a
Aberrant metaphases (−Gap/+Gap)
PP + ER
RCCb (%)
Vehicle control
−
−
6–18
0.0/0.0
0.0
100.0
625
1.78/0.31
−
6–18
1.0/1.5
0.0
86.5
AMK
1,250
3.56/0.63
−
6–18
0.5/0.5
0.0
77.8
2,500
7.13/1.25
−
6–18
2.0/2.0
0.0
54.6
MMC
0.04
−
−
6–18
22.0/22.5*
0.0
−
0.0
Vehicle control
−
+
6–18
0.5/0.5
0.0
100.00
1,250
3.56/0.63
+
6–18
0.5/0.5
0.0
92.1
AMK
2,500
7.13/1.25
+
6–18
0.5/1.0
0.0
86.2
5,000
14.25/2.50
+
6–18
1.0/1.5
0.0
69.1
CPA
10.00
−
+
6–18
27.0/27.5*
0.0
−
0.0
Vehicle control
−
−
24–0
0.5/0.5
0.0
100.0
625
1.78/0.31
−
24–0
0.5/1.0
0.0
75.1
AMK
1,250
3.56/0.63
−
24–0
1.5/2.0
0.0
72.5
2,500
7.13/1.25
−
24–0
2.5/2.5
0.0
53.9
MMC
0.04
−
−
24–0
27.0/28.0*
0.0
−
Abbreviations: PP: polyploidy; ER: endoreduplication; RCC: relative cell counts; MMC: mitomycin C; CPA: cyclophosphamide.
aTreatment time-recovery time.
bRCC (relative cell count) equals (no. of treated cells/no. of control cells) × 100 (%).
*Significantly different from the vehicle control at .
