Phytochemical Analysis and Antimicrobial, Antinociceptive, and Anti-Inflammatory Activities of Two Chemotypes of Pimenta pseudocaryophyllus (Myrtaceae)

Table 4

Minimum inhibitory concentration (𝜇g mL⁻¹) of extracts, fractions, and essential oils from leaves of P. pseudocaryophyllus (𝐸)-methyl isoeugenol and citral chemotypes, and of the citral substance.

Microorganisms	EEM	HFM	DFM	EAFM	AFM	EOM	EEC	HFC	DFC	EAFC	AFC	EOC	Citral
													(Sigma)
Gram (+) bacteria
S.aureus	1000	1000	500	1000	1000	>1000	500	>1000	250	500	1000	250	125
ATCC 6538
S.aureus	1000	1000	500	1000	1000	>1000	500	>1000	500	1000	1000	125	125
ATCC 25923
S.epidermidis	1000	1000	250	1000	1000	>1000	1000	1000	250	500	1000	125	250
ATCC 12229
M.luteus	1000	1000	250	1000	1000	>1000	500	>1000	250	500	1000	125	125
ATCC 9341
M.roseus	1000	1000	500	500	1000	>1000	500	1000	500	500	1000	125	125
ATCC 1740
B.cereus	1000	1000	250	1000	500	>1000	500	1000	250	500	1000	62.5	125
ATCC 14579
B.atrophaeus	1000	1000	500	1000	1000	>1000	500	>1000	500	500	1000	125	125
ATCC 6633
Gram (−) bacteria
E.coli	>1000	>1000	>1000	1000	>1000	>1000	>1000	>1000	>1000	1000	1000	>1000	>1000
ATCC 8739
E.coli	>1000	>1000	>1000	1000	>1000	>1000	>1000	>1000	>1000	1000	1000	>1000	1000
ATCC 11229
E.aerogenes	>1000	>1000	>1000	1000	>1000	>1000	>1000	>1000	>1000	1000	>1000	>1000	>1000
ATCC 13048
E.cloacae	>1000	>1000	>1000	1000	>1000	>1000	>1000	>1000	>1000	1000	1000	>1000	1000
HMA/FTA502
S.marcescens	>1000	>1000	>1000	>1000	>1000	>1000	>1000	>1000	>1000	1000	>1000	>1000	>1000
ATCC 14756
Salmonella spp.	1000	1000	1000	1000	1000	>1000	1000	>1000	1000	1000	1000	>1000	500
ATCC 19430
P.aeruginosa	1000	1000	1000	1000	1000	>1000	1000	1000	1000	500	1000	>1000	1000
ATCC 27483
P.aeruginosa	1000	1000	1000	1000	500	>1000	1000	1000	1000	1000	1000	>1000	1000
SPM1
Fungi
C.albicans 02	125	62.5	>500	31.2	62.5	>500	62.5	500	250	31.2	62.5	500	125
C.parapsilosis 11A	62.5	62.5	500	15.6	31.2	>500	31.2	250	250	31.2	31.2	500	250
C.parapsilosis	62.5	62.5	500	31.2	62.5	>500	31.2	250	250	31.2	31.2	500	125
ATCC 22019
C.neoformans var.	15.6	15.6	>500	>500	15.6	125	15.6	250	500	7.8	15.6	15.6	7.8
neoformans
ATCC 28957
C.neoformans var.	125	31.2	500	>500	7.8	250	7.8	62.5	500	15.6	>500	62.5	15.6
neoformans L2
C.neoformans var.	125	31.2	>500	>500	31.2	250	15.6	500	500	15.6	>500	62.5	31.2
gatti L1

EE_M: ethanol extract of the (𝐸)-methyl isoeugenol chemotype. HF_M: hexane fraction of the (𝐸)-methyl isoeugenol chemotype. DF_M: dichloromethane fraction of the (𝐸)-methyl isoeugenol chemotype. EAF_M: ethyl acetate fraction of the (𝐸)-methyl isoeugenol chemotype. AF_M: aqueous fraction of the (𝐸)-methyl isoeugenol chemotype. EO_M: essential oil of the (𝐸)-methyl isoeugenol chemotype. EE_C: ethanol extract of the citral chemotype. HF_C: hexane fraction of the citral chemotype. DF_C: dichloromethane fraction of the citral chemotype. EAF_C: ethyl acetate fraction of the citral chemotype. AF_C: aqueous fraction of the citral chemotype. EO_C: essential oil of the citral chemotype.