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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 423690, 12 pages
http://dx.doi.org/10.1155/2012/423690
Research Article

Antidiabetic Effect of an Active Components Group from Ilex kudingcha and Its Chemical Composition

Key Laboratory of Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, China

Received 31 August 2011; Revised 21 November 2011; Accepted 22 November 2011

Academic Editor: Ching Liang Hsieh

Copyright © 2012 Chengwu Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The leaves of Ilex kudingcha are used as an ethnomedicine in the treatment of symptoms related with diabetes mellitus and obesity throughout the centuries in China. The present study investigated the antidiabetic activities of an active components group (ACG) obtained from Ilex kudingcha in alloxan-induced type 2 diabetic mice. ACG significantly reduced the elevated levels of serum glycaemic and lipids in type 2 diabetic mice. 3-Hydroxy-3-methylglutaryl coenzyme A reductase and glucokinase were upregulated significantly, while fatty acid synthetase, glucose-6-phosphatase catalytic enzyme was downregulated in diabetic mice after treatment of ACG. These findings clearly provided evidences regarding the antidiabetic potentials of ACG from Ilex kudingcha. Using LC-DAD/HR-ESI-TOF-MS, six major components were identified in ACG. They are three dicaffeoylquinic acids that have been reported previously, and three new triterpenoid saponins, which were the first time to be identified in Ilex kudingcha. It is reasonable to assume that antidiabetic activity of Ilex kudingcha against hyperglycemia resulted from these six major components. Also, synergistic effects among their compounds may exist in the antidiabetic activity of Ilex kudingcha.