A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells
Figure 6
Effect of KIOM-MA on (a) translocation of the NF-κB (p65) subunit into the nucleus and (b) release of IκBα into the cytosol upon LPS stimulation. The cells were treated with LPS alone or with LPS and KIOM-MA for 1 hour. The level of IκBα protein in the cytosol and NF-κB (p65) protein present in the cytosol and nucleus was determined by the Western blot analysis using anti-IκBα or anti-NF-κB p65 antibody, as described in Section 2. β-actin and TBP were used for cytosolic and nuclear control protein, respectively. The protein value in the cells stimulated by LPS without KIOM-MA treatment was set to 1.00 and the effect by treatment was quantitated. The experiment was repeated three times independently.
