Effects of Electroacupuncture of Different Frequencies on the Release Profile of Endogenous Opioid Peptides in the Central Nerve System of Goats

Table 2

β-EP immunoactivities induced by different frequencies in the CNS (mean ± SD, 𝑛=7).

Nuclei; and areas	Sham control	0 Hz	2 Hz	40 Hz	60 Hz	100 Hz	Correlation coefficients
ACB	10.57 ± 1.48^d	9.25 ± 0.44^d	12.21 ± 2.00^c	21.01 ± 1.80^b	27.61 ± 1.91^a	18.70 ± 1.21^b	0.891**
CAU	20.90 ± 1.99^c	20.84 ±3.87^c	24.05 ± 2.99^bc	36.60 ± 2.17^a	36.99 ± 2.28^a	26.81 ± 2.41^b	0.835**
SA	21.04 ± 2.03^d	20.65 ± 3.58^d	29.58 ± 2.66^c	42.08 ± 4.24^b	49.52 ± 3.02^a	31.25 ± 3.21^c	0.893**
AMY	18.46 ± 2.45^d	18.21 ± 3.33^d	32.95 ± 4.16^c	53.15 ± 2.74^ab	54.59 ± 3.09^a	48.86 ± 3.66^b	0.840**
SON	29.31 ± 1.52^c	28.88 ± 2.37^c	38.18 ± 5.30^b	53.13 ± 2.60^a	52.68 ± 2.10^a	51.25 ± 1.87^a	0.783**
ARC	33.45 ± 2.11^a	33.42 ± 2.34^a	35.13 ± 2.02^a	26.83 ± 1.13^bc	23.76 ± 2.74^c	28.83 ± 2.63^b	0.795**
VMH	19.81 ± 2.10^e	18.86 ± 1.79^e	34.41 ± 2.82^d	56.30 ± 3.77^b	61.17 ± 2.35^a	47.94 ± 1.66^c	0.897**
HB	17.57 ± 2.42^c	16.12 ± 1.63^c	18.44 ± 1.49^c	22.59 ± 2.05^b	26.37 ± 1.20^a	27.46 ± 1.30^a	0.647**
PAG	8.40 ± 0.96^d	8.22 ± 1.23^d	19.04 ± 1.74^c	28.82 ± 3.82^b	33.70 ± 3.36^a	30.18 ± 3.55^ab	0.871**
DR	18.63 ± 1.83^d	17.82 ± 2.34^d	29.07 ± 1.94^c	34.06 ± 3.09^ab	38.43 ± 3.45^a	31.10 ± 3.09^bc	0.808**
LC	14.90 ± 2.21^d	13.72 ± 2.95^d	21.01 ± 2.37^c	30.13 ± 2.66^b	35.14 ± 2.42^a	30.35 ± 1.80^b	0.855**
PBN	7.65 ± 1.19^b	7.54 ± 1.44^b	8.69 ± 0.99^b	14.07 ± 2.85^a	14.92 ± 1.96^a	7.99 ± 1.15^b	0.790**
NRM	17.37 ± 2.20^c	16.52 ± 2.23^c	17.93 ± 1.21^c	23.50 ± 1.65^b	30.25 ± 2.91^a	24.89 ± 1.76^b	0.779**
SOL	11.50 ± 1.02^e	11.35 ± 0.88^e	23.41 ± 2.46^d	33.83 ± 3.64^c	49.46 ± 1.42^a	43.59 ± 2.17^b	0.928**
SCD	7.25 ± 1.13^d	6.80 ± 0.26^d	10.91 ± 1.00^c	14.42 ± 1.18^b	17.72 ± 1.03^a	10.30 ± 1.26^c	0.795**

ACB: nucleus accumbens, SA: septal area, CAU: caudate nucleus, AMY: amygdala, SON: supraoptic nucleus, ARC: arcuate nucleus, LC: locus ceruleus, PAG: periaqueductal gray, DR: dorsal raphe nucleus, PBN: parabrachial nucleus, HB: habenular nucleus, NRM: nucleus raphe, SOL: solitary nucleus, VMH: ventromedial nucleus of hypothalamus magnus, SCD: spinal cord dorsal horn.