It Is Not Just Folklore: The Aqueous Extract of Mung Bean Coat Is Protective against Sepsis

Figure 5

Characterization of active components of MBC extract. (a, b) HPLC fractionation of MBC extract. MBC extract was fractioned by HPLC, and each fraction was screened for activity in inhibiting endotoxin-induced HMGB1 release. Note that two major UV absorption peaks (I and II) were detected at the wavelengths of 254, 280, and 360 nm (a) and coeluted with the activities to inhibit endotoxin-induced HMGB1 release (b). (c, d) Mass spectrometry analysis of HPLC peaks. The MS spectrum of the MBC HPLC peak II was identical to that of the commercially obtained isovitexin standard.