Figure 1: In GBM cells, ISCADOR Q changes the expression of cancer associated genes. (a) PCR-based microarray expression analysis. LNT-229 cells were treated with ISCADOR Q (100 μg/mL) or were left untreated. 24 h later, the cells were harvested, mRNA was prepared, reverse transcribed into cDNA and used for microarray-based expression analysis on a panel of 96 migration-/invasion-angiogenesis-involved genes. The best downregulated genes with a threshold are shown. (b) RT-PCR-based validation of differentially regulated genes detected in (a) at 12 or 24 h of ISCADOR Q treatment ( , SEM). (c) TGF- ELISA of ISCADOR Q treated LNT-229 cells. The cells were treated with ISCADOR Q (100 μg/mL, 24 h) followed by incubation in serum-free medium for 48 h. Supernatants were harvested and TGF-β 1 or TGF-β 2 was quantified using ELISA ( , SEM). (d) The cells were treated with ISCADOR Q (100 μg/mL, 12 or 24 h). Supernatants were harvested 48 h later. Angiopoietin-1 content was analyzed using ELISA ( , SEM). (e) The cells were treated as in (c). Supernatants were harvested and VEGF content was analyzed using ELISA ( , SEM). (f) Contingency table analysis of the PCR-based microarray expression data together with the corresponding genes of the REMBRANDT database. Nominal scaled response variable (genes significantly up- (1), non- (0), or down- (−1) regulated in glioblastomas versus normal brain tissue expression pattern in the REMBRANDT database) and nominal explanatory variable (same gene up- or downregulated in our PCR-based microarray expression analysis after ISCADOR treatment) were analysed and subsequently tested by the likelihood ratio test ( ).