Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 5

Research Article

Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1 -Induced NF-κB Activation in Canine Articular Chondrocytes

Figure 5

Effect of botanical extracts on signaling proteins and cartilage-specific transcription factor SOX-9 in chondrocytes. Serum-starved chondrocytes ( cells/mL) were cultured for 24 h and then treated with 10 ng/mL IL-1β for 48 h, botanical extracts (each 10 μg/mL) for 72 h, or pretreated with botanical extracts (10 μg/mL each) for 24 h and then cotreated with 10 ng/mL IL-1β for 48 h or left untreated and evaluated after 72 h. Results of western blot analysis revealed down-regulation of Shc (I), ERK1/2 (II), and SOX-9 (III) in chondrocytes with IL-1β. Cotreatment of chondrocytes preincubated with botanical extracts and IL-1β relieved the IL-1β-induced inhibition of Shc (I), ERK1/2 (II), and SOX-9 (III). In untreated and in positive control cultures, expression of Shc, ERK1/2, and SOX-9 were equally strong in chondrocytes (I–III). Expression of β-actin was not affected by IL-1β and/or botanical extracts (IV). Data shown are representative of three independent experiments. Treatments: C (untreated control); IL-1β; RC (Rosa canina); SA (Salix alba); UD (Urtica dioica).

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