Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1-Induced NF-κB Activation in Canine Articular Chondrocytes
Figure 6
Botanical extracts block the IL-1β-induced phosphorylation and nuclear translocation of p65 in chondrocytes. Western blot analysis of IL-1β-treated nuclear extracts. Serum-starved chondrocytes ( cells/mL) were pretreated with botanical extracts (10 μg/ML each) for 4 hours followed by cotreatment with 10 ng/mL IL-1β and botanical extracts for 1 h. Some chondrocyte cultures remained either untreated or were treated with 10 μg/mL botanical extracts (each alone) or with 10 ng/mL IL-1β alone for 1 h. Nuclear extracts were probed for phospho p65, (I) by western blot analysis using antibodies to p65, phospho-specific p65, and PARP (II, control). Treatment of chondrocytes with IL-1β (10 ng/mL) revealed a clear increase in expression of phospho-p65 in the nuclear extracts (I). Co-treatment of chondrocytes with botanical extracts (all three) completely abolished the IL-1β-dependent activation of phospho p65 in the nucleus (I). Synthesis of PARP remained unaffected in nuclear extracts (II). Data shown are representative of three independent experiments. Treatments: C (untreated control); IL-1β; RC (Rosa canina); SA (Salix alba); UD (Urtica dioica).
