Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1𝜷-Induced NF-κB Activation in Canine Articular Chondrocytes

Figure 7

Botanical extracts inhibit IL-1β-induced nuclear translocation of NF-κB in chondrocytes. Chondrocyte cultures either served as controls (a) or were treated with IL-1β alone for 10 min (b) or cotreated with botanical extracts for 10 min and then cotreated with IL-1β for 1 h (c–e) before immunolabeling with NF-κB antibodies and FITC-coupled secondary antibodies. In control cells anti-NF-κB labeling was restricted to the cytoplasm (a). Cells treated with IL-1β alone revealed nuclear translocation of NF-κB (b) that was partly inhibited by cotreatment with botanical extracts (c–e). (a–e): ×160. Data shown are representative of three independent experiments.