Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 8

Research Article

Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1 -Induced NF-κB Activation in Canine Articular Chondrocytes

Figure 8

Effects of botanical extracts on the kinetics of IκBα by IL-1β in chondrocytes. Effect of botanical extracts on IL-1β-induced degradation of IκBα. Western blot analysis with IL-1β-treated cytoplasmic extracts. Serum-starved chondrocytes ( cells/mL) were treated with IL-1β (10 ng/mL) for 0, 10, 30 and 60 min. Other cultures were initially treated with botanical extracts (10 μg/mL) for 4 h and then cotreated with IL-1β (10 ng/mL) for the indicated times or left untreated (medium controls). The cytoplasmic extracts were prepared, fractionated on SDS-PAGE, and electroblotted onto nitrocellulose membranes. Western blot analysis was performed with anti-IκBa and anti-β-actin (control). IL-1β caused IκBα degradation in cultures as early as 10 min after treatment. In cotreated chondrocytes with each botanical extracts the degradation of IκBα was not observed (I–III). Synthesis of β-actin remained unaffected (IV). Data shown are representative of three independent experiments. Treatments: medium (controls); IL-1β; RC (Rosa canina); SA (Salix alba); UD (Urtica dioica).

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