Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats

Table 1

Summary of the sequences of RT-PCR primers, the appropriate annealing temperature used in experiments, and product size.

Genes	Primer	Annealing temperature (°C)	Product size (bp)
GAPDH	S 5′-TCAACGGCACAGTCAAGG-3′	54	299
	A 5′-ACCAGTGGATGCAGGGAT-3′
RST	S 5′-CACAGTGGGCAGACTGGACCAGAGC-3′	57	412
	A 5′-CCAAGGATGAGCGAAGGA-3′
OAT1	S 5′-TAATACCGAAGAGCCATACGA-3′	56	358
	A 5′-TCCTGCTGCTGTTGATTCTGC-3′
MRP4	S 5′-AAATCGGAATCTCCTGTCTG −3′	56	203
	A 5′-TATGAGGTCGGCGAATGA-3′
ABCG2	S 5′- TAGCAGCAAGGAAAGAC−3′	54	835
	A 5′-TGATGACAGAACGAGGTA-3′
XDH	S 5′-CTTTGCGAAGGATGAGGTT-3′	58	412
	A 5′-CACTCGGACTACGATTCTGTT-3′
CPT1	S 5′-CCACGAAGCCCTCAAACAGA-3′	57	315
	A 5′-AGCACCTTCAGCGAGTAGCG-3′
OCTN2	S 5′-AGGTTTGGTCGCAAGAATG-3′	56	458
	A 5′-AACTCACTGGGATCGAAGAT-3′
PPARα	S 5′-GGCTCGGAGGGCTCTGTCATC-3′	56	655
	A 5′-ACATGCACTGGCAGCAGTGGA-3′
SREBP-1	S 5′-GGAGCGAGCATTGAACTGTAT-3′	58	344
	A 5′-GGGCAGCCTTGAAGGAGTA-3′
PGC-1β	S 5′-GGTACAGCTCATTCGCTACAT-3′	58	210
	A 5′-TAGGGCTTGCTAACATCACA-3′