Figure 3: Bromelain LC-MS/MS and DYGAVNEVK peptide quantification. Individual mice were treated with Br (one–12 mg/kg i.p. injection) and peripheral blood was collected via cardiac puncture at 3, 6, 12, and 24 hrs and processed. Plasma was collected and processed, and filtrate was analyzed by LC-MS/MS. The total number of spectra of peptide “DYGAVNEVK” from Br protein FB1035 precursor was identified in sera peaking at 6 hrs over the 24 hr time course.