Polygonum cuspidatum Extract Induces Anoikis in Hepatocarcinoma Cells Associated with Generation of Reactive Oxygen Species and Downregulation of Focal Adhesion Kinase

Figure 3

PCE induced anoikis in hepatocarcinoma cells. (a) Bel-7402 cells were treated with different dose of PCE for 24 h in Poly-HEMA coated plates, stained with EthD-1, and observed under fluorescence microscope (×100). (b) The fluorescence of EthD-1 absorbed by Bel-7402 cells was detected with fluorescence microplate reader and expressed as mean ± SD. (c) PCE-treated Bel-7402 cells and Hepa 1–6 cells were stained with Annexin V-FITC/PI and detected in FACScalibur flow cytometer. Data illustrated are from three separate experiments. *𝑃<0.05, versus control group. **𝑃<0.01, versus control group.

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