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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 623723, 11 pages
http://dx.doi.org/10.1155/2012/623723
Research Article

Antibacterial Activities of Selected Cameroonian Plants and Their Synergistic Effects with Antibiotics against Bacteria Expressing MDR Phenotypes

1Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
2Transporteurs Membranaires, Chimioresistance et Drug Design, UMR-MD1, IFR 88, Université de la Méditerranée, Aix-Marseille II, Marseille, France

Received 20 October 2011; Accepted 13 December 2011

Academic Editor: Namrita Lall

Copyright © 2012 Stephen T. Lacmata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present work was designed to assess the antibacterial properties of the methanol extracts of some Cameroonian medicinal plants and the effect of their associations with currently used antibiotics on multidrug resistant (MDR) Gram-negative bacteria overexpressing active efflux pumps. The antibacterial activities of twelve methanol extracts of medicinal plants were evaluated using broth microdilution. The results of this test showed that three extracts Garcinia lucida with the minimal inhibitory concentrations (MIC) varying from 128 to 512  g/mL, Garcinia kola (MIC of 256 to 1024  g/mL), and Picralima nitida (MIC of 128 to 1024  g/mL) were active on all the twenty-nine studied bacteria including MDR phenotypes. The association of phenylalanine arginine -naphthylamide (PA N or efflux pumps inhibitor) to different extracts did not modify their activities. At the concentration of MIC/2 and MIC/5, the extracts of P. nitida and G. kola improved the antibacterial activities of some commonly used antibiotics suggesting their synergistic effects with the tested antibiotics. The results of this study suggest that the tested plant extracts and mostly those from P. nitida, G. lucida and G. kola could be used alone or in association with common antibiotics in the fight of bacterial infections involving MDR strains.