Tumor Microenvironment Varies under Different TCM ZHENG Models and Correlates with Treatment Response to Herbal Medicine
Figure 3
Correlation between microenvironment and tumor growth under different ZHENG conditions. (a) The levels of CAF- and TAM-derived secreted cytokines, SDF-1 and CCL5, in tumor from the Shi-Re, Pi-Xu, and Xue-Yu ZHENG models were evaluated with ELISA assay. Data are expressed as the mean ± SE. (b and c) IHC staining for CXCR4 and CCR5 on sections of tumors from ZHENG models was performed and representative photos are shown in (b). The positive rates of CXCR4 and CCR5 protein in tumors with indicated ZHENG are shown in (c). (d) Effect of ZHENG on tumor growth in a subcutaneously transplanted tumor model. Xenograft tumor model combined with ZHENG model was established as described in Figure 2(a). Mean volumes of tumors from each group were measured. Mean ± standard deviation was determined for 10 mice in each group.
