Tumor Microenvironment Varies under Different TCM ZHENG Models and Correlates with Treatment Response to Herbal Medicine
Figure 4
Response of tumors under different ZHENG conditions to herbal medicine treatments. (a) IHC staining for vimentin, a-SMA, and CD68 in tumors from the indicated group was performed for evaluating CAF proliferative activities and TAM infiltration, respectively. CAF proliferative activity and TAM infiltration were quantitatively evaluated as described in Figure 2(b). (b) The levels of SDF-1 and CCL5 in indicated tumors were evaluated with ELISA assay. Data are expressed as the mean ± SE. (c) IHC staining for CXCR4 and CCR5 on sections of indicated tumors. The positive rates of CXCR4 and CCR5 protein in tumors with indicated ZHENG was calculated. (d) Effect of herbal medicine on subcutaneously transplanted tumor with different ZHENG. The growth curves for each are shown. n.s.: not significant.
