Fisetin Inhibits Hyperglycemia-Induced Proinflammatory Cytokine Production by Epigenetic Mechanisms

Figure 3

Effect of fisetin on HAT and HDAC activity as well as p300 and acetylated CBP/p300 levels in HG-treated THP-1 cells. Cells were harvested after 48 h of fisetin treatment and nuclear lysates were prepared. Samples were analyzed for determination of HAT (a) and HDAC activity (b). Results are shown as mean ± SD for 3 different experiments. ^††𝑃<0.01 compared to NG; *𝑃<0.05; **𝑃<0.01 compared to HG. (c) After nuclear protein extraction, p300 and acetylated CBP/p300 levels were evaluated by western blot. The immunoblots shown here are representative of 3 independent experiments.

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