Figure 2: (a) TRPC6 mRNA expression changes at different time points. Note: 1, 4, 7-8 h; 2, 5, 8–24 h; 3, 6, 9–48 h. (b) GAPDH amplification map. (c) TRPC6 amplification map. Note: there was a slight peak in 3rd and 4th samples, ct value reached 34.77, 34.69, indicated that there was Trpc6 mRNA expression in the 3rd and 4th samples, but the expression is low.