Mechanism of Earthquake Simulation as a Prenatal Stressor Retarding Rat Offspring Development and Chinese Medicine Correcting the Retardation: Hormones and Gene-Expression Alteration

Table 1

QC of RNA extraction and slides experiment (A sample is qualified only when 2100 RIN ≥ 7.0 and 28S/18S ≥ 0.7).

Group	QC of RNA							QC of slides
	Con. (𝜇g/μL)	Vol. (μL)	Total (μg)	A260/A280	2100 Result		Result	CV (%)*	Detection rate (%)
					RIN	28S/18S
ESG	0.168	50	8.41	1.88	9.4	1.7	Qualified	3.91	69.50
	1.366	30	40.98	1.93	9.5	1.8	Qualified	4.85	62.96
	0.246	50	12.29	1.90	9.4	1.7	Qualified	6.70	72.13
HG	0.134	50	6.69	1.81	9.4	1.8	Qualified	4.76	70.40
	0.138	50	6.92	1.82	9.4	1.8	Qualified	4.90	72.30
	0.372	50	18.58	1.86	9.5	1.6	Qualified	4.89	69.43
CG	0.185	50	9.27	1.91	9.4	1.7	Qualified	6.33	61.09
	0.595	50	29.75	1.93	9.4	1.7	Qualified	5.70	70.68
	0.355	25	8.87	1.85	9.3	1.6	Qualified	4.39	65.52

*CV = SD/Mean × 100%.