Effects of Different Electroacupuncture Scheduling Regimens on Murine Bone Tumor-Induced Hyperalgesia: Sex Differences and Role of Inflammation
Figure 1
Graphs illustrating (a) tumor-induced mechanical hyperalgesia from postimplantation day (PID) 3 to 21 in male and female mice as measured using a von Frey filament applied 10 times to the hind paw. Pronounced hyperalgesia is evident as early as PID 3 and extends through PID 21 in both male and female animals (/group) as compared to their saline controls (/group, *, **, ***, and ****); (b) a comparison of the effect of electroacupuncture treatment given twice per week beginning on PID 3 (EA-2X/3) on tumor-induced mechanical hyperalgesia in Bulb/c osteosarcoma male mice (/group) compared to C3H fibrosarcoma male mice (/group). Electroacupuncture produced a significant decrease in tumor-induced hyperalgesia in both mouse strains (*, **, ***). Note that Bulb/c mice were used for the behavioral and immunohistochemical experiments, while C3H mice were used for the microperfusion experiments in this study; (c) the effect of EA administered once per week starting on PID 7 on tumor-induced hyperalgesia; and (d) the effect of sham acupuncture administered once per week starting at day 7 on tumor-induced hyperalgesia. EA-1X/7, but not sham EA, produces an anti-nociceptive effect only in female animals and only on PID 7. Data is presented as the mean % response ± SEM.
(a) MF Saline versus MF Tumor
(b) M Fibrosarcoma (FS) versus M Osteosarcoma (OS)
