Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress
Figure 1
Changes of the levels of sperm ROS, MMP, and motility in rats under oxidative stress without and with TSL-A or GA feeding. (a) Change of ROS level in sperm. Sperms from different group were incubated with DCFH2-DA as an indicator for ROS; (b) change of the MMP level in sperm. Sperms from different group were incubated with DiCO6 as an indicator for MMP; (c) sperm motility of different group. Rats were randomly divided into five groups: vehicle group, H2O2 group, the H2O2 treatment plus TSL-A feeding (TSL-A), H2O2 treatment, and H2O2 treatment plus GA feeding (GA). Both the ROS and MMP levels of sperm were measured by flow cytometry. The sperm motility was determined by microscope. The symbols “” and “” represent the increased and decreased ROS and MMP level compared with that of vehicle group, respectively. compared with vehicle group.
