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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 682720, 11 pages
Research Article

A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain

1Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary’s Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea
2Department of Radiology, Incheon St. Mary’s Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea
3Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA

Received 30 January 2012; Revised 30 March 2012; Accepted 31 March 2012

Academic Editor: Ilkay Erdogan Orhan

Copyright © 2012 Yun-Young Sunwoo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

PET Imaging Data Analysis and Quantitative Analysis of Infarct Volume. We confirm whether infarct volume was associated with brain metabolism for one day post-MCAo. At 1 day after MCAo model, rat (n=5) was injected intravenously F-18 FDG into the caudal vein. PET imaging produces 6 coronal image planes separated by 3 mm in stereotatix apparatus on pinpoint marker. After sacrifice at scanned rats, the brain was removed and sectioned into four equally spaced (2 mm) coronal blocks using a rodent brain matrix. These sections were stained 2% TTC. The injured area of brain slices were quantified using Meta-Morph program. The total infarct volume for each slice was calculated by summation of all brain slices. To assess changes in metabolism induced by MCAo, average ROI in each hemisphere were identified in images of the coronal brain sections. The mean SUV of F-18 FDG in ROI was calculated as the averaged nCi/cc after calibration for both ipsilateral and contralateral insulated areas in the same images. The radioacitivity in the contralateral area was used as a reference to normalize data obtained in the ipslateral area, and the DUR was calculated. The total DUR for each imaging was calculated by summation of all brain imaging.

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