Fractionation of an Extract of Pluchea odorata Separates a Property Indicative for the Induction of Cell Plasticity from One That Inhibits a Neoplastic Phenotype
Figure 2
(a) Induction of apoptosis of HL60 cells by F2.1–F2.9. 1 × 105 cells/mL were seeded in 24-well plates and incubated with 10 μg/mL of each fraction for 72 h. Then, cells were double stained with Hoechst 33258 and propidium iodide and examined under the microscope with UV light connected to a DAPI filter. Nuclei with morphological changes which indicated cell death were counted, and the percentages of dead cells were calculated. Significance was calculated in comparison to control (Co). (b) Antiproliferative effect of F3.3 and F3.6: 1 × 105 cells/mL were seeded into 24-well plate, incubated with 2 and 10 μg/mL of each fraction, and the percentage of proliferation was calculated relative to solvent treated control within a 24 h period. Experiments were performed in triplicate. Asterisks indicate significance compared to untreated control () and error bars indicate ±SD. (c) Thin layer chromatography (TLC) of F2.6 and F3.1–F3.10; Mobile phase: TLC system 2. Detection: visible light with ASR.
