Figure 4: Effects of AKH extract on the development of AD induced by house dust mite-induced AD in NC/Nga mice. NC/Nga mice were used as a model of house dust mite induced AD. The mice were divided into four groups: untreated (Cont), Biostir-AD plus vehicle treatment (Biostir), Biostir-AD plus Protopic treatment (Protopic), and Biostir-AD plus AKH extract treatment (AKH). Clinical features (a) and dermatitis scores (b) were assessed using the criteria described in Section 2. The features were monitored once a week for 4 weeks. Values are expressed as the means ± SEM ( ). *P < 0.05 versus Biostir-AD treated mice.