Figure 5: Effect of AKH extract on histological features of house dust mite-induced AD in NC/Nga mice.The skin and ear tissues of untreated (Cont), Biostir-AD plus vehicle-treated (Biostir), Biostir-AD plus Protopic-treated (Protopic), and Biostir-AD plus AKH extract-treated (AKH) mice were excised, fixed with 10% formaldehyde, and embedded in paraffin, and thin sections were made. The histological features of the skin and ear lesions were stained with hematoxylin and eosin and the back skin sections were stained with toluidine blue. Scale bars: 100 μm.