Research Article
Rats Born to Mothers Treated with Dexamethasone 15 cH Present Changes in Modulation of Inflammatory Process
Table 1
Immunohistochemistry used markers (adapted from KAWAKAMI, 2011) [
29].
| Marker | Cell | Source | Molecular target | Clone | Origin/target (species) | Dilution |
| Anti-CD54 | Activated endothelial cell | Serotec | ICAM 1 | 1A29 | Mouse-rat | 1 : 10 (5 μg/mL) | Anti-CD18 | Leukocytes | Serotec | Beta-2 integrin | WT.3 | Mouse-rat | 1 : 10 (5 μg/mL) | Anti-CD163 | Activated macrophages | Serotec | Surface glycoprotein (ED2) | ED2 | Mouse-rat | 1 : 10 (5 μg/mL) | Anti-MAC 387 | Monocytes, macrophages, and polymorphonuclear | AbCAM | Intracytoplasmatic protein (calprotectin) | polyclonal | Rabbit-rat | 1 : 20 (50 μg/mL) |
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